Alexa Fluor 647-conjugated AffiniPure Goat Anti-Mouse IgG, F(ab)2 Fragment Specific
Format:
IgG
Target Specificity:
IgG (F(ab')2)
Cross-Adsorption (MinX):
no cross-adsorbtion
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab')2/Fab portion of mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against the Fc portion of mouse IgG or against non-immunoglobulin serum proteins. The antibody may cross-react with immunogloublins from other species.
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Clonality:
Polyclonal
Isotype:
Whole IgG
Buffer:
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Purity:
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Form:
Freeze-dried solid
Antibody Type:
Secondary Antibody
Application Dilute:
1:100 - 1:800 for most applications
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