Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Form:
Lyophilized
Sequence:
Cys22-Arg221
Target:
2B4/CD244/SLAMF4
Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human 2B4 is greater than 90% as determined by SEC-HPLC.
Immobilized Human 2B4, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA.
Loaded Human CD48, hFc Tag on ProA-Biosensor can bind Human 2B4, His Tag with an affinity constant of 0.51 µM as determined in BLI assay (Gator Prime).
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