Human 2B4/CD244/SLAMF4 Protein (2B4-HM201)

Product Overview

• Article Name: Human 2B4/CD244/SLAMF4 Protein (2B4-HM201)
• Biozol Catalog Number: KAB-2B4-HM201
• Supplier Catalog Number: 2B4-HM201
• Alternative Catalog Number: KAB-2B4-HM201-100UG, KAB-2B4-HM201-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: 2B4, CD244 antigen, CD244, h2B4, NAIL, NKR2B4, Nmrk, SLAMF4

Product Description

Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Cys22-Arg221.

Product Properties

• Molecular Weight: The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: Q9BZW8
• Buffer: Lyophilized from 0.22µm filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Cys22-Arg221
• Target: 2B4/CD244/SLAMF4

Images

Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human 2B4 is greater than 95% as determined by SEC-HPLC.

Immobilized Human CD48, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.

Loaded Human CD48, His Tag on Anti-His-Biosensor can bind Human 2B4, hFc Tag with an affinity constant of 5.79 nM as determined in BLI assay (Gator Prime).