Biotinylated Human 2B4/CD244/SLAMF4 Protein (2B4-HM401B)

Product Overview

• Article Name: Biotinylated Human 2B4/CD244/SLAMF4 Protein (2B4-HM401B)
• Biozol Catalog Number: KAB-2B4-HM401B
• Supplier Catalog Number: 2B4-HM401B
• Alternative Catalog Number: KAB-2B4-HM401B-100UG, KAB-2B4-HM401B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: 2B4, CD244 antigen, CD244, h2B4, NAIL, NKR2B4, Nmrk, SLAMF4

Product Description

Recombinant Biotinylated Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys22-Arg221.

Product Properties

• Molecular Weight: The protein has a predicted MW of 24.9 kDa. Due to glycosylation, the protein migrates to 48-70 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: Q9BZW8
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Cys22-Arg221
• Target: 2B4/CD244/SLAMF4

Images

Biotinylated Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human 2B4 is greater than 95% as determined by SEC-HPLC.

Immobilized Biotinylated Human 2B4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).

Loaded Human CD48, hFc Tag on ProA-Biosensor can bind Biotinylated Human 2B4, His-Avi Tag with an affinity constant of 24.90 nM as determined in BLI assay (Gator Prime).