Human ACE2/ACEH Protein (ACE-HM101)

Product Overview

• Article Name: Human ACE2/ACEH Protein (ACE-HM101)
• Biozol Catalog Number: KAB-ACE-HM101
• Supplier Catalog Number: ACE-HM101
• Alternative Catalog Number: KAB-ACE-HM101-100UG, KAB-ACE-HM101-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: ACE-2, ACEH, ACE2

Product Description

Recombinant Human ACE2/ACEH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln18-Ser740.

Product Properties

• Molecular Weight: The protein has a predicted MW of 84.7 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: Q9BYF1
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Gln18-Ser740
• Target: ACE2/ACEH

Images

Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC

SARS-COV-2 Spike RBD captured on Protein A chip, can bind Human ACE2, His Tag with an affinity constant of 11.9nM as determined in a SPR assay (Biacore T200).

Immobilized Human ACE2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA (QC Test).