Biotinylated Human ACE2/ACEH Protein (ACE-HM501B)

Product Overview

• Article Name: Biotinylated Human ACE2/ACEH Protein (ACE-HM501B)
• Biozol Catalog Number: KAB-ACE-HM501B
• Supplier Catalog Number: ACE-HM501B
• Alternative Catalog Number: KAB-ACE-HM501B-100UG, KAB-ACE-HM501B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: ACE-2, ACEH, ACE2

Product Description

Recombinant Biotinylated Human ACE2/ACEH Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Gln18-Ser740.

Product Properties

• Molecular Weight: The protein has a predicted MW of 111.2 kDa. Due to glycosylation, the protein migrates to 115-125 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)-Avi
• UniProt: Q9BYF1
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE
• Form: Lyophilized
• Sequence: Gln18-Ser740
• Target: ACE2/ACEH

Images

Biotinylated Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized SARS-COV-2 Spike S, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA.

Loaded Biotinylated Human ACE2, hFc-Avi Tag on Streptavidin-Biosensor can bind SARS-COV-2 Spike S, hFc-Avi Tag with an affinity constant of 0.17 µM as determined in BLI assay (Gator Prime).