Recombinant Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminus.It contains Ile21-Thr503.
Molecular Weight:
The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-70 kDa based on Bis-Tris PAGE result.
Tag:
C-His
UniProt:
XP_045223825.1
Buffer:
Lyophilized from 0.22 µm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Ile21-Thr503
Target:
Alkaline Phosphatase (Placental type)/ALPP
The purity of Biotinylated Cynomolgus ALPP is greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus ALPP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Biotinylated Cynomolgus ALPP, His Tag- with an affinity constant of 82.54 nM as determined in SPR assay (Biacore T200).
Loaded Anti-Alkaline Phosphatase Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus ALPP, His Tag with an affinity constant of 0.13 µM as determined in BLI assay (Gator Prime).
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