Human Alkaline phosphatase (Intestinal type) / ALPI Protein (APE-HM101)

Product Overview

• Article Name: Human Alkaline phosphatase (Intestinal type) / ALPI Protein (APE-HM101)
• Biozol Catalog Number: KAB-APE-HM101
• Supplier Catalog Number: APE-HM101
• Alternative Catalog Number: KAB-APE-HM101-100UG, KAB-APE-HM101-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: IAP, Akp2, ALPI, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, HOPS, MGC161443, TNAP, TNSALP

Product Description

Recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Val20-Asp503.

Product Properties

• Molecular Weight: The protein has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Bis-Tris PAGE result.
• Tag: N-His-Flag
• UniProt: P09923
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Val20-Asp503
• Target: Alkaline phosphatase (Intestinal type)/ALPI

Images

The purity of Human Alkaline phosphatase (Intestinal type) is greater than 95% as determined by SEC-HPLC.

Human Alkaline phosphatase (Intestinal type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline phosphatase (Intestinal type) , His Tag with an affinity constant of 1.58 uM as determined in SPR assay (Biacore T200).

Anti-Alkaline Phosphatase Antibody, hFc Tag on ProA-Biosensor, can bind Human Alkaline Phosphatase, His Tag with an affinity constant of 0.87 µM as determined in BLI assay (Gator).