Biotinylated Human Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (APE-HM102B)

Product Overview

• Article Name: Biotinylated Human Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (APE-HM102B)
• Biozol Catalog Number: KAB-APE-HM102B
• Supplier Catalog Number: APE-HM102B
• Alternative Catalog Number: KAB-APE-HM102B-100UG, KAB-APE-HM102B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: Alkaline phosphatase, PLAP-1, ALPP, PLAP

Product Description

Recombinant Biotinylated Human Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminus.It contains Ile23-Asp506.

Product Properties

• Molecular Weight: The protein has a predicted MW of 53.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: P05187
• Buffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ile23-Asp506
• Target: Alkaline Phosphatase (Placental type)/ALPP

Images

Biotinylated Human Alkaline Phosphatase (Placental type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Alkaline Phosphatase (Placental type) is greater than 95% as determined by SEC-HPLC.

Immobilized Biotinylated Human Alkaline Phosphatase, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.

Loaded Anti-Alkaline Phosphatase Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human Alkaline Phosphatase, His Tag with an affinity constant of 0.58 nM as determined in BLI assay (Gator Prime).