Human Alkaline Phosphatase (Germ type) / ALPG Protein (APE-HM203)

Product Overview

• Article Name: Human Alkaline Phosphatase (Germ type) / ALPG Protein (APE-HM203)
• Biozol Catalog Number: KAB-APE-HM203
• Supplier Catalog Number: APE-HM203
• Alternative Catalog Number: KAB-APE-HM203-100UG, KAB-APE-HM203-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: Alkaline Phosphatase, ALP-1, Alkaline phosphatase, placental-like, GCAP, PLAP-like, ALPG, ALPPL, ALPPL2

Product Description

Recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ile20-Asp503.

Product Properties

• Molecular Weight: The protein has a predicted MW of 79.33 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: P10696
• Buffer: Lyophilized from 0.22µm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ile20-Asp503
• Target: Alkaline Phosphatase (Germ type)/ALPG

Images

Human Alkaline Phosphatase (Germ type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Alkaline Phosphatase (Germ type) is greater than 95% as determined by SEC-HPLC.

Immobilized Human Alkaline Phosphatase (Germ Type), hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA.

Loaded Biotinylated Anti-Alkaline Phosphatase Ab., hFc-Avi Tag on Streptavidin-Biosensor can bind Human Alkaline Phosphatase(Germ Type), hFc Tag with an affinity constant of 59.00 pM as determined in BLI assay (Gator Prime).