Non-biotinylated Human ANGPT2/Angiopoietin-2 Protein (APN-HM401)

Product Overview

• Article Name: Non-biotinylated Human ANGPT2/Angiopoietin-2 Protein (APN-HM401)
• Biozol Catalog Number: KAB-APN-HM401
• Supplier Catalog Number: APN-HM401
• Alternative Catalog Number: KAB-APN-HM401-100UG, KAB-APN-HM401-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: AGPT2, ANG2, ANG-2, angiopoietin 2, Angiopoietin-2, angiopoietin-2a, angiopoietin-2B, angiopoitin 2, ANGPT2, Tie2-ligand

Product Description

Recombinant Human ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Lys275-Phe496.

Product Properties

• Molecular Weight: The protein has a predicted MW of 28.4 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
• Tag: N-His-Avi
• UniProt: O15123
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Lys275-Phe496
• Target: ANGPT2/Angiopoietin-2

Images

Human ANGPT2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Human TIE2, His Tag immobilized on CM5 Chip can bind Human ANGPT2, His Tag with an affinity constant of 2.38 uM as determined in SPR assay (Biacore T200).

Immobilized Human ANGPT2, His Tag at 2ug/ml (100ul/well). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA (QC Test).

The purity of Human ANGPT2 is greater than 95% as determined by SEC-HPLC.