Biotinylated Human APRIL/TNFSF13 Trimer Protein (APR-HM410B)

Product Overview

• Article Name: Biotinylated Human APRIL/TNFSF13 Trimer Protein (APR-HM410B)
• Biozol Catalog Number: KAB-APR-HM410B
• Supplier Catalog Number: APR-HM410B
• Alternative Catalog Number: KAB-APR-HM410B-100UG, KAB-APR-HM410B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: CD256, TALL2, TALL-2, TNFSF13, APRIL, APRILFLJ57090, TRDL1, TRDL-1, ZTNF2, 2310026N09Rik

Product Description

Recombinant Biotinylated Human APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag, Avi tag and Flag Tag at the N-Terminus.It contains Lys112-Leu250 trimer design.

Product Properties

• Molecular Weight: The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
• Tag: N-His-Avi-Flag
• UniProt: O75888
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Form: Lyophilized
• Sequence: Lys112-Leu250
• Target: APRIL/TNFSF13

Images

Biotinylated Human APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human APRIL Trimer is greater than 90% as determined by SEC-HPLC.

Immobilized Human BCMA, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 11.9ng/ml determined by ELISA.

Loaded Human TACI, hFc Tag on ProA-Biosensor can bind Biotinylated Human APRIL (Trimer), His-Avi-Flag Tag with an affinity constant of 0.67nM as determined in BLI assay (Gator Prime).