Biotinylated Human AXL Protein (AXL-HM401B)

Product Overview

• Article Name: Biotinylated Human AXL Protein (AXL-HM401B)
• Biozol Catalog Number: KAB-AXL-HM401B
• Supplier Catalog Number: AXL-HM401B
• Alternative Catalog Number: KAB-AXL-HM401B-100UG, KAB-AXL-HM401B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: Axl, UFO, AXL oncogene, AXL, ARK, JTK11, Tyro7, AI323647, EC 2.7.10, EC 2.7.10.1

Product Description

Recombinant Biotinylated Human AXL Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Pro449.

Product Properties

• Molecular Weight: The protein has a predicted MW of 48.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P30530
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ala26-Pro449
• Target: AXL

Images

Biotinylated Human AXL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human AXL is greater than 95% as determined by SEC-HPLC.

Immobilized Biotinylated Biotinylated Human Axl, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA.

Loaded Anti-Axl Ab., hFc Tag on ProA-Biosensor can bind Human Axl, His-Avi Tag with an affinity constant of 87.30 nM as determined in BLI assay (Gator Prime).