Human B7-1/CD80 Protein (B71-HM280)

Product Overview

• Article Name: Human B7-1/CD80 Protein (B71-HM280)
• Biozol Catalog Number: KAB-B71-HM280
• Supplier Catalog Number: B71-HM280
• Alternative Catalog Number: KAB-B71-HM280-100UG, KAB-B71-HM280-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD80 molecule, B7, B71, B7-1, BB1, CD28LG1, CD80, B7.1, CD28LG1, LAB7

Product Description

Recombinant Human B7-1/CD80 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Val35-Asn242.

Product Properties

• Molecular Weight: The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: P33681
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Val35-Asn242
• Target: B7-1/CD80

Images

Human B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human B7-1 is greater than 95% as determined by SEC-HPLC.

Immobilized Human CTLA-4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.

Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 43.61 uM as determined in SPR assay (Biacore T200).