Non-biotinylated Human B7-1/CD80 Protein (B71-HM480)

Product Overview

• Article Name: Non-biotinylated Human B7-1/CD80 Protein (B71-HM480)
• Biozol Catalog Number: KAB-B71-HM480
• Supplier Catalog Number: B71-HM480
• Alternative Catalog Number: KAB-B71-HM480-100UG, KAB-B71-HM480-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD80 molecule, B7, B71, B7-1, BB1, CD28LG1, CD80, B7.1, CD28LG1, LAB7

Product Description

Recombinant Human B7-1/CD80 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val35-Asn242.

Product Properties

• Molecular Weight: The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P33681
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Val35-Asn242
• Target: B7-1/CD80

Images

Human B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of- Human B7-1 is greater than 95% as determined by SEC-HPLC.

Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).

Immobilized Human CTLA-4, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48ug/ml determined by ELISA (QC Test).