Cynomolgus B7-2/CD86 Protein, Ultra Low Endotoxin (B72-CM186-UL)

Product Overview

• Article Name: Cynomolgus B7-2/CD86 Protein, Ultra Low Endotoxin (B72-CM186-UL)
• Biozol Catalog Number: KAB-B72-CM186-UL
• Supplier Catalog Number: B72-CM186-UL
• Alternative Catalog Number: KAB-B72-CM186-UL-100UG, KAB-B72-CM186-UL-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Monkey
• Alternative Names: CD86 molecule, CD86, B70, B7-2 antigen, B72, B7-2, BU63, FUN-1, LAB72, MGC34413, CD28LG2

Product Description

Recombinant Cynomolgus B7-2/CD86 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Tyr25-His239.

Product Properties

• Molecular Weight: The protein has a predicted MW of 26.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: A0A2K5TTJ8
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Tyr25-His239
• Target: B7-2/CD86

Images

Cynomolgus B7-2, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 0.102 uM as determined in SPR assay (Biacore T200).

Immobilized Cynomolgus B7-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA (QC Test).

Cynomolgus B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus B7-2 is greater than 95% as determined by SEC-HPLC.