Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with monomeric Fc tag and Flag tag at the N-Terminus.It contains Thr141-Leu285 trimer design.
Molecular Weight:
The protein has a predicted MW of 74.9 kDa same as Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Thr141-Leu285
Target:
BAFF/TNFSF13B/CD257
Human BAFF (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BAFF (Trimer) is greater than 95% as determined by SEC-HPLC.
Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF (Trimer) , hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
Immobilized Human BAFF Trimer, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
Loaded Human BAFFR, His Tag on Anti-His-Biosensor can bind Human BAFF (Trimer), monomeric Fc-Flag Tag with an affinity constant of 0.98 nM as determined in BLI assay (Gator Prime).
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