Biotinylated Human BAFFR/TNFRSF13C Protein (BAF-HM40RB)

Product Overview

• Article Name: Biotinylated Human BAFFR/TNFRSF13C Protein (BAF-HM40RB)
• Biozol Catalog Number: KAB-BAF-HM40RB
• Supplier Catalog Number: BAF-HM40RB
• Alternative Catalog Number: KAB-BAF-HM40RB-100UG, KAB-BAF-HM40RB-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: CD268, BAFFR, BR3, TNFRSF13C, BROMIX, CVID4, prolixin

Product Description

Recombinant Biotinylated Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser7-Ala71.

Product Properties

• Molecular Weight: The protein has a predicted MW of 9.5 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: Q96RJ3
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE
• Form: Lyophilized
• Sequence: Ser7-Ala71
• Target: BAFFR/TNFRSF13C

Images

Biotinylated Human BAFFR on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

Immobilized Anti-BAFFR Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 5.5ng/ml determined by ELISA.

Immobilized Human BAFF (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 0.42ug/ml determined by ELISA (QC Test).

Loaded Biotinylated Human BAFFR, His-Avi Tag on Streptavidin-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 0.20 nM as determined in BLI assay (Gator Prime).

Loaded Biotinylated Human BAFFR, His-Avi Tag on Anti-His-Biosensor can bind Anti-BAFFR Ab., hFc Tag with an affinity constant of 18.5 pM as determined in BLI assay (Gator Prime).