Non-biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM541)

Product Overview

• Article Name: Non-biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM541)
• Biozol Catalog Number: KAB-BB4-HM541
• Supplier Catalog Number: BB4-HM541
• Alternative Catalog Number: KAB-BB4-HM541-100UG, KAB-BB4-HM541-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD137, TNFRSF9, 4-1BB ligand receptor, 41BB, 4-1BB, CDw137, FLJ43501, ILA, T cell antigen ILA

Product Description

Recombinant Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Leu24-Gln186.

Product Properties

• Molecular Weight: The protein has a predicted MW of 45.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)-Avi
• UniProt: Q07011
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Leu24-Gln186
• Target: 4-1BB/TNFRSF9

Images

Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human 4-1BB is greater than 95% as determined by SEC-HPLC.

Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.

Loaded Non-biotinylated Human 4-1BB, hFc-Avi Tag on ProA-Biosensor can bind Human 4-1BB Ligand (Trimer), His Tag with an affinity constant of 0.18 nM as determined in BLI assay (Gator Prime).