Biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM541B)
Biozol Catalog Number:
KAB-BB4-HM541B
Supplier Catalog Number:
BB4-HM541B
Alternative Catalog Number:
KAB-BB4-HM541B-100UG, KAB-BB4-HM541B-500UG
Manufacturer:
KactusBio
Category:
Proteine/Peptide
Species Reactivity:
Human
Conjugation:
Biotin
Alternative Names:
CD137, TNFRSF9, 4-1BB ligand receptor, 41BB, 4-1BB, CDw137, FLJ43501, ILA, T cell antigen ILA
Recombinant Biotinylated Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Leu24-Gln186.
Molecular Weight:
The protein has a predicted MW of 45.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Leu24-Gln186
Target:
4-1BB/TNFRSF9
Biotinylated Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human 4-1BB , hFc Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human 4-1BB Ligand (Trimer) , His Tag with the EC50 of 14.5ng/ml determined by ELISA.
Loaded Biotinylated Human 4-1BB, hFc-Avi Tag on ProA-Biosensor can bind Human 4-1BB Ligand (Trimer), His Tag with an affinity constant of 0.21 nM as determined in BLI assay (Gator Prime).
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