Biotinylated Human BCMA/TNFRSF17 Protein (BCM-HM117B)

Product Overview

• Article Name: Biotinylated Human BCMA/TNFRSF17 Protein (BCM-HM117B)
• Biozol Catalog Number: KAB-BCM-HM117B
• Supplier Catalog Number: BCM-HM117B
• Alternative Catalog Number: KAB-BCM-HM117B-100UG, KAB-BCM-HM117B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: CD269, TNFRSF17, BCMA, BCM, TNFRSF13A

Product Description

Recombinant Biotinylated Human BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Ala54.

Product Properties

• Molecular Weight: The protein has a predicted MW of 8.9 kDa. Due to glycosylation, the protein migrates to 12 kDa and 15-17 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: Q02223
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE
• Form: Lyophilized
• Sequence: Met1-Ala54
• Target: BCMA/TNFRSF17

Images

Biotinylated Human BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Anti-BCMA Antibody, hFc Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA.

Immobilized Biotinylated Human BCMA, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFF, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).

Immobilized Human APRIL (Trimer), His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human BCMA, His Tag with the EC50 of 38.2ng/ml determined by ELISA.

Loaded Anti-BCMA antibody on ProA-Biosensor can bind Biotinylated Human BCMA, His-Avi Tag with an affinity constant of 4.09 nM as determined in BLI assay (Gator Prime).