Recombinant Cynomolgus B7-H7/HHLA2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile21-Asn345.
Molecular Weight:
The protein has a predicted MW of 38.31 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Tag:
C-His
UniProt:
XP_005548285.2
Buffer:
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Ile21-Asn345
Target:
B7-H7/HHLA2
The purity of Cynomolgus B7-H7 is greater than 95% as determined by SEC-HPLC.
Cynomolgus B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus B7-H7, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD28H, hFc Tag with an affinity constant of 7.86 nM as determined in SPR assay (Biacore T200).
Loaded Cynomolgus B7-H7, His Tag on Anti-His-Biosensor can bind Human CD28H, hFc Tag with an affinity constant of 1.72 nM as determined in BLI assay (Gator Prime).
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