Biotinylated Human B7-H4 Protein (BH7-HM174B)

Product Overview

• Article Name: Biotinylated Human B7-H4 Protein (BH7-HM174B)
• Biozol Catalog Number: KAB-BH7-HM174B
• Supplier Catalog Number: BH7-HM174B
• Alternative Catalog Number: KAB-BH7-HM174B-100UG, KAB-BH7-HM174B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: B7H4, B7-H4, B7S1, B7h.5, B7x, FLJ22418, VTCN1, PRO1291

Product Description

Recombinant Biotinylated Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe29-Ala258.

Product Properties

• Molecular Weight: The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: Q7Z7D3
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Phe29-Ala258
• Target: B7-H4

Images

Biotinylated Human B7-H4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human B7-H4 is greater than 95% as determined by SEC-HPLC.

Immobilized Biotinylated Human B7-H4, His Tag at 2ug/ml (100ul/Well) on streptavidin(5ug/ml) precoated plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test).

Loaded Anti-B7-H4 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human B7-H4, His-Avi Tag with an affinity constant of 6.90 nM as determined in BLI assay (Gator Prime).