Human B7-H2/ICOSLG Protein (BH7-HM272)

Product Overview

• Article Name: Human B7-H2/ICOSLG Protein (BH7-HM272)
• Biozol Catalog Number: KAB-BH7-HM272
• Supplier Catalog Number: BH7-HM272
• Alternative Catalog Number: KAB-BH7-HM272-100UG, KAB-BH7-HM272-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD275, B7H2, B7-H2, B7RP-1, GL50, ICOSL, ICOS-L, ICOSLG, B7RP1, LICOS, ICOS ligand, B7-H2B7-related protein 1, B7RP-1, B7RP1B7-like protein Gl50

Product Description

Recombinant Human B7-H2/ICOSLG Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Asp19-Ser258.

Product Properties

• Molecular Weight: The protein has a predicted MW of 53.4 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: O75144
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Asp19-Ser258
• Target: B7-H2/ICOSLG

Images

Human B7-H2 captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).

Immobilized Human ICOS, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test).

The purity of Human B7-H2 is greater than 95% as determined by SEC-HPLC.

Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.