Biotinylated Human B7-H7/HHLA2 Protein (BH7-HM477B)

Product Overview

• Article Name: Biotinylated Human B7-H7/HHLA2 Protein (BH7-HM477B)
• Biozol Catalog Number: KAB-BH7-HM477B
• Supplier Catalog Number: BH7-HM477B
• Alternative Catalog Number: KAB-BH7-HM477B-100UG, KAB-BH7-HM477B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: B7H7, B7-H7, HHLA2, B7 Homolog 7

Product Description

Recombinant Biotinylated Human B7-H7/HHLA2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile23-Asn344.

Product Properties

• Molecular Weight: The protein has a predicted MW of 39.88 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: Q9UM44
• Buffer: Supplied as 0.22 µm filtered solution in PBS (pH 7.4).
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Liquid
• Sequence: Ile23-Asn344
• Target: B7-H7/HHLA2

Images

The purity of Biotinylated Human B7-H7 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human B7-H7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Biotinylated Human B7-H7, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD28H, hFc Tag (Cat. CD8-HM28H) with the EC50 of 4.9ng/ml determined by ELISA.

Loaded Biotinylated Human B7-H7, His-Avi Tag on Streptavidin-Biosensor can bind Human CD28H, hFc Tag (Cat. CD8-HM28H) with an affinity constant of 5.28 nM as determined in BLI assay (Gator Prime).