Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Glu47-Lys279
Target:
B7-H2/ICOSLG
Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse B7-H2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 36.2ng/ml determined by ELISA.
The purity of Mouse B7-H2 is greater than 95% as determined by SEC-HPLC.
Mouse B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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