Non-biotinylated Human BTLA Protein (BTL-HM401)

Product Overview

• Article Name: Non-biotinylated Human BTLA Protein (BTL-HM401)
• Biozol Catalog Number: KAB-BTL-HM401
• Supplier Catalog Number: BTL-HM401
• Alternative Catalog Number: KAB-BTL-HM401-100UG, KAB-BTL-HM401-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD272, BTLA, BTLA1, FLJ16065, MGC129743

Product Description

Recombinant Human BTLA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys31-Ser150.

Product Properties

• Molecular Weight: The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: Q7Z6A9
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Lys31-Ser150
• Target: BTLA

Images

Human BTLA-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human BTLA-is greater than 95% as determined by SEC-HPLC.

Anti-BTLA antibody captured on Protein A chip, can bind Human BTLA, His Tag with an affinity constant of 0.99nM as determined in a SPR assay (Biacore T200).

Immobilized Human BTLA, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.

Immobilized Human BTLA, His Tag at 2ug/ml (100ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test).