Human EpCAM/TROP1 Protein, Ultra Low Endotoxin (CAM-HM2EP-UL)

Product Overview

• Article Name: Human EpCAM/TROP1 Protein, Ultra Low Endotoxin (CAM-HM2EP-UL)
• Biozol Catalog Number: KAB-CAM-HM2EP-UL
• Supplier Catalog Number: CAM-HM2EP-UL
• Alternative Catalog Number: KAB-CAM-HM2EP-UL-100UG, KAB-CAM-HM2EP-UL-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD326, EGP, EGP-2, EGP314, EGP40, EpCAM, ACSTD1, DIAR5, gp40, HNPCC8, MOC31, TACST-1, TACSTD1, TROP1, ESA, KSA, MIC18, MK1

Product Description

Recombinant Human EpCAM/TROP1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln24-Lys265.

Product Properties

• Molecular Weight: The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: P16422
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Gln24-Lys265
• Target: EpCAM/TROP1

Images

Human EpCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..

The purity of Human EpCAM is greater than 95% as determined by SEC-HPLC.

Human EpCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human AGR-2, His Tag with an affinity constant of 6.91 uM as determined in SPR assay (Biacore T200).

Immobilized Human EpCAM, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA (QC Test).