Human UltraPro Mutant CD19 Protein (CD1-HM129)

Product Overview

• Article Name: Human UltraPro Mutant CD19 Protein (CD1-HM129)
• Biozol Catalog Number: KAB-CD1-HM129
• Supplier Catalog Number: CD1-HM129
• Alternative Catalog Number: KAB-CD1-HM129-100UG, KAB-CD1-HM129-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD19, B4, CVID3, Leu-12, MGC12802

Product Description

Recombinant Human UltraPro Mutant CD19 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro20-Pro278 with site mutations.

Product Properties

• Molecular Weight: The protein has a predicted MW of 29.93 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: P15391
• Buffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Pro20-Pro278 with site mutations
• Target: CD19

Images

Immobilized Human UltraPro Mutant CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for FMC63, mFc Tag with the EC50 of 9.5ng/ml determined by ELISA.

Immobilized Human UltraPro Mutant CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.

Immobilized Human UltraPro Mutant CD19, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Denintuzumab, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.

Immobilized Human UltraPro Mutant CD19, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.

The purity of Human UltraPro Mutant CD19 is greater than 95% as determined by SEC-HPLC.

Human UltraPro Mutant CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Coltuximab on ProA-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 9.48 nM as determined in BLI assay (Gator Prime).

Loaded FMC63 on MFC-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 3.80 nM as determined in BLI assay (Gator Prime).

Loaded Inebilizumab on ProA-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 3.97 nM as determined in BLI assay (Gator Prime).

Loaded Denintuzumab on ProA-Biosensor can bind Human UltraPro Mutant CD19, His Tag with an affinity constant of 8.05 nM as determined in BLI assay (Gator Prime).