Human CD155/PVR Protein (CD1-HM255)

Product Overview

• Article Name: Human CD155/PVR Protein (CD1-HM255)
• Biozol Catalog Number: KAB-CD1-HM255
• Supplier Catalog Number: CD1-HM255
• Alternative Catalog Number: KAB-CD1-HM255-100UG, KAB-CD1-HM255-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD155, HVED, NECL5, Necl-5, nectin-like 5, PVR, PVS, Tage4, PVSFLJ25946, FLJ25946

Product Description

Recombinant Human CD155/PVR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Trp21-Asn343.

Product Properties

• Molecular Weight: The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: P15151
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Form: Lyophilized
• Sequence: Trp21-Asn343
• Target: CD155/PVR

Images

Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 uM as determined in SPR assay (Biacore T200).

Human CD155 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Human CD96, mFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA.

Immobilized Human TIGIT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.38ug/ml determined by ELISA.

The purity of Human CD155 is greater than 90% as determined by SEC-HPLC.