Non-biotinylated Human IL-2 R beta/CD122 Protein (CD1-HM422)

Product Overview

• Article Name: Non-biotinylated Human IL-2 R beta/CD122 Protein (CD1-HM422)
• Biozol Catalog Number: KAB-CD1-HM422
• Supplier Catalog Number: CD1-HM422
• Alternative Catalog Number: KAB-CD1-HM422-100UG, KAB-CD1-HM422-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD122, IL-15 R beta, IL-2 R beta, IL-2R subunit beta, IL2RB, P70-75, p75, RP5-1170K4.6, IL-15 R beta, IL-2 R beta, IL-2R subunit beta

Product Description

Recombinant Human IL-2 R beta/CD122 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Asp239.

Product Properties

• Molecular Weight: The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: NP_000869
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ala27-Asp239
• Target: IL-2 R beta/CD122

Images

Human IL-2 R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.

Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9nM as determined in SPR assay (Biacore T200).

Human IL-2 R beta, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).

Immobilized Human IL-2 R beta, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, hFc Tag with the EC50 of 43.7ng/ml determined by ELISA (QC Test).