Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM105)

Product Overview

• Article Name: Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM105)
• Biozol Catalog Number: KAB-CD3-HM105
• Supplier Catalog Number: CD3-HM105
• Alternative Catalog Number: KAB-CD3-HM105-100UG, KAB-CD3-HM105-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD3, CD3e, CD3E, CD3d, T3D, CD3D, CD3E&CD3D, CD3 delta&CD3 epsilon

Product Description

Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with His tag at the C-Terminus of CD3D. It contains Asp23-Asp126 (CD3E) and Phe22-Ala105 (CD3D).

Product Properties

• Molecular Weight: The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 28-38 kDa and 25 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: P07766
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)
• Target: CD3E&CD3D/CD3 epsilon&CD3 delta

Images

The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

Human CD3E&CD3D, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200).

Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Human CD3E&CD3D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.

Immobilized Human CD3E&CD3D, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 39.3ng/ml determined by ELISA (QC Test).