Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM157)

Product Overview

• Article Name: Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM157)
• Biozol Catalog Number: KAB-CD3-HM157
• Supplier Catalog Number: CD3-HM157
• Alternative Catalog Number: KAB-CD3-HM157-100UG, KAB-CD3-HM157-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD3, CD3 epsilon, CD3epsilon, CD3epsilon, CD3 epsilon, CD3E, CD3e, CD3 gamma, CD3gamma, CD3gamma, CD3 gamma, CD3G, CD3g, CD3epsilon&CD3gamma, CD3 epsilon&CD3 gamma, CD3 epsilon & CD3 gamma, CD3epsilon&CD3gamma, CD3 epsilon&CD3 gamma, CD3 epsilon & CD3 gamma, CD3E&CD3G, CD3E & CD3G, CD3 E & CD3 G, CD3e&CD3g, CD3e & CD3g, CD3 e & CD3 g

Product Description

Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with His tag at the C-Terminus of CD3G.It contains Asp23-Asp126 (CD3E) and Gln23-Ser116 (CD3G).

Product Properties

• Molecular Weight: The protein has a predicted MW of 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: P07766
• Buffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G)
• Target: CD3E&CD3G/CD3 epsilon&CD3 gamma

Images

Human CD3E&CD3G, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).

Human CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.

Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Foralumab, hFc Tag with the EC50 of 36.2 ng/ml determined by ELISA.

Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2 ng/ml determined by ELISA (QC Test).