Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc (IgG1) at the C-Terminus of CD3E and CD3D.It contains Asp23-Asp126(CD3E) and Phe22-Ala105(CD3D).
Molecular Weight:
The protein has a predicted MW of 37.8 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation, the protein migrates to 48-58 kDa (CD3E) and 42-48 kDa (CD3D) based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Form:
Lyophilized
Sequence:
Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)
Target:
CD3E&CD3D/CD3 epsilon&CD3 delta
The purity of Human CD3E&CD3D was greater than 90% as determined by SEC-HPLC.
Immobilized Human CD3E&CD3D, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA.
Immobilized Tebentafusp, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD3E&CD3D, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human CD3E&CD3D, hFc Tag at 2ug/ml (100ul/Well). Dose response curve for Anti-CD3E&CD3D Ab.2, mFc Tag with the EC50 of 12.9ng/ml determined by ELISA (QC Test).
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