Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus of CD3D.It contains Asp23-Asp126 (CD3E) and Phe22-Ala105 (CD3D).
Molecular Weight:
The protein has a predicted MW of 17.9 kDa (CD3E) and 18.52 kDa (CD3D). Due to glycosylation, the protein migrates to 23-27 kDa and 32-40 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Asp23-Asp126 (CD3E) & Phe22-Ala105 (CD3D)
Target:
CD3E&CD3D/CD3 epsilon&CD3 delta
Immobilized Biotinylated Human CD3E&CD3D, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 0.11ug/ml determined by ELISA.
Immobilized Biotinylated Human CD3E&CD3D, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA.
The purity of Biotinylated Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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