Biotinylated Human CD40/TNFRSF5 Protein (CD4-HM440B)

Product Overview

• Article Name: Biotinylated Human CD40/TNFRSF5 Protein (CD4-HM440B)
• Biozol Catalog Number: KAB-CD4-HM440B
• Supplier Catalog Number: CD4-HM440B
• Alternative Catalog Number: KAB-CD4-HM440B-100UG, KAB-CD4-HM440B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: CD40, CD40L receptor, TNFRSF5, CD40 antigen, CD40 molecule, CDw40, MGC9013,

Product Description

Recombinant Biotinylated Human CD40/TNFRSF5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu21-Arg193.

Product Properties

• Molecular Weight: The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P25942
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Glu21-Arg193
• Target: CD40/TNFRSF5

Images

Biotinylated Human CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD40 is greater than 95% as determined by SEC-HPLC.

Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.

Immobilized Anti-CD40 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD40, His Tag with the EC50 of 31.3ng/ml determined by ELISA.

Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test).