Human CD58 Protein (CD5-HM208)

Product Overview

• Article Name: Human CD58 Protein (CD5-HM208)
• Biozol Catalog Number: KAB-CD5-HM208
• Supplier Catalog Number: CD5-HM208
• Alternative Catalog Number: KAB-CD5-HM208-100UG, KAB-CD5-HM208-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: Lymphocyte function-associated antigen 3,Ag3,CD58,LFA3

Product Description

Recombinant Human CD58 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Phe29-Arg215.

Product Properties

• Molecular Weight: The protein has a predicted MW of 48.1 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: AAH05930
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Phe29-Arg215
• Target: CD58

Images

Human CD58 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CD58 is greater than 95% as determined by SEC-HPLC.

Human CD2, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD58, hFc Tag with an affinity constant of 15.48 nM as determined in SPR assay (Biacore T200).

Immobilized Human CD2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD58, hFc Tag with the EC50 of 0.32ug/ml determined by ELISA (QC Test).