Non-biotinylated Human Fc gamma RIIA/CD32a (H167) Protein, Ultra Low Endotoxin (CDA-HM432-UL)
Biozol Catalog Number:
KAB-CDA-HM432-UL
Supplier Catalog Number:
CDA-HM432-UL
Alternative Catalog Number:
KAB-CDA-HM432-UL-100UG, KAB-CDA-HM432-UL-500UG
Manufacturer:
KactusBio
Category:
Proteine/Peptide
Species Reactivity:
Human
Alternative Names:
FcgammaRIIA, Fc gammaRIIA, Fcgamma RIIA, Fc gamma RIIA, FcgammaRIIA, Fc gammaRIIA, Fcgamma RIIA, Fc gamma RIIA, FcgRIIA, Fc gRIIA, Fcg RIIA, Fc g RIIA, FCG2, FCGR2, FCGR2A, FCGR2A1, FCRIIA, fcRII-a, FCG2, CD32A
Recombinant Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln34-Ile218(H167).
Molecular Weight:
The protein has a predicted MW of 23.4 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gln34-Ile218(H167)
Target:
Fc gamma RIIA/CD32a
Human Fc gamma RIIA (H167) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of-Human Fc gamma RIIA (H167) is greater than 95% as determined by SEC-HPLC.
Human Fc gamma RIIA (H167) , His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 1.53 uM as determined in SPR assay (Biacore T200) (QC Test).
Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIA (H167) , His Tag with an affinity constant of 0.73 uM as determined in SPR assay (Biacore T200).
Loaded Human Fc gamma RIIA (H167), His Tag on Anti-His-Biosensor, can bind Rituximab with an affinity constant of 0.71 µM as determined in BLI assay (Gator).
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