Non-biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein (CDB-HM401)

Product Overview

• Article Name: Non-biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein (CDB-HM401)
• Biozol Catalog Number: KAB-CDB-HM401
• Supplier Catalog Number: CDB-HM401
• Alternative Catalog Number: KAB-CDB-HM401-100UG, KAB-CDB-HM401-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: Fc gamma RII, Fc gamma RIIB, Fc gamma RIIC, CD32, CD32b, CD32c, CDw32, FCG2, IGFR2, CD32A, FCG2 , FcGR, FCGR2, FCGR21, FCGR2A, FcgammaRIIB, Fc gammaRIIB, Fcgamma RIIB, Fc gamma RIIB

Product Description

Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala46-Pro217.

Product Properties

• Molecular Weight: The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P31994
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ala46-Pro217
• Target: Fc gamma RIIB/C (CD32b/c)

Images

The purity of Human Fc gamma RIIB/C is greater than 95% as determined by SEC-HPLC.

Human Fc gamma RIIB/C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIB/C, His Tag with an affinity constant of 3.46 uM as determined in SPR assay (Biacore T200).

Human Fc gamma RIIB/C, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 16.72 uM as determined in SPR assay (Biacore T200).

Loaded Human Fc gamma RIIB/C, His Tag on Anti-His-Biosensor can bind Rituximab with an affinity constant of 3.09 µM as determined in BLI assay (Gator Prime).