Fc gamma RII, Fc gamma RIIB, CD32, CD32b, CDw32, FCG2, IGFR2, CD32A, FCG2 , FcGR, FCGR2, FCGR21, FCGR2A, FcgammaRIIB, Fc gammaRIIB, Fcgamma RIIB, Fc gamma RIIB
Recombinant Mouse Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr40-Arg217.
Molecular Weight:
The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Tag:
C-His
UniProt:
NP_001070657.1
Buffer:
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Form:
Lyophilized
Sequence:
Thr40-Arg217
Target:
Fc gamma RIIB/CD32b
Mouse Fc gamma RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Fc gamma RIIB is greater than 90% as determined by SEC-HPLC.
OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIIB, His Tag with an affinity constant of 0.280 uM as determined in SPR assay (Biacore T200).
Loaded OKT3 on ProA-Biosensor can bind Mouse Fc gamma RIIB,His Tag with an affinity constant of 1.99 µM as determined in BLI assay (Gator).
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