Recombinant Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Met113-Leu261 trimer design.
Molecular Weight:
The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Met113-Leu261
Target:
CD40 Ligand/TNFSF5
Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Immobilized Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8ng/ml determined by ELISA.
Loaded Human CD40, hFc Tag on ProA-Biosensor can bind Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), His-Flag Tag with an affinity constant of 0.23 µM as determined in BLI assay (Gator Prime).
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