SARS-COV-2 Spike RBD (N501Y) Protein (COV-VM1BY)

Product Overview

• Article Name: SARS-COV-2 Spike RBD (N501Y) Protein (COV-VM1BY)
• Biozol Catalog Number: KAB-COV-VM1BY
• Supplier Catalog Number: COV-VM1BY
• Alternative Catalog Number: KAB-COV-VM1BY-100UG, KAB-COV-VM1BY-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Virus
• Alternative Names: S protein RBD, S glycoprotein RBD, Spike protein RBD, sars-cov-2

Product Description

Recombinant SARS-COV-2 Spike RBD (N501Y) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(N501Y).

Product Properties

• Molecular Weight: The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: QHD43416.1
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Arg319-Phe541(N501Y)
• Target: Spike RBD

Images

Human ACE2 captured on Protein A chip, can bind SARS-COV-2 Spike RBD (N501Y) , His Tag with an affinity constant of 1.74nM as determined in a SPR assay (Biacore T200).

SARS-COV-2 Spike RBD (N501Y) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized SARS-COV-2 Spike RBD (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test).

The purity of SARS-COV-2 Spike RBD (N501Y) is greater than 95% as determined by SEC-HPLC.