Non-biotinylated SARS-COV-2 Spike S1 Protein (COV-VM5S1)

Product Overview

• Article Name: Non-biotinylated SARS-COV-2 Spike S1 Protein (COV-VM5S1)
• Biozol Catalog Number: KAB-COV-VM5S1
• Supplier Catalog Number: COV-VM5S1
• Alternative Catalog Number: KAB-COV-VM5S1-100UG, KAB-COV-VM5S1-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Virus
• Alternative Names: S1 protein, S glycoprotein Subunit1, S1 protein, Spike protein S1, Spike, sars-cov-2

Product Description

Recombinant SARS-COV-2 Spike S1 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Gln14-Arg683.

Product Properties

• Molecular Weight: The protein has a predicted MW of 102.6 kDa. Due to glycosylation, the protein migrates to 130-140 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)-Avi
• UniProt: QHD43416.1
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Form: Lyophilized
• Sequence: Gln14-Arg683
• Target: Spike S1

Images

SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of SARS-COV-2 Spike S1 is greater than 90% as determined by SEC-HPLC.

SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200).

Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA (QC Test).

Loaded SARS-COV-2 Spike S1, hFc Tag on ProA-Biosensor can bind Non-biotinylated Human ACE2, His Tag with an affinity constant of 0.66 nM as determined in BLI assay (Gator Prime).