Human M-CSF/CSF-1 Protein, Ultra Low Endotoxin (CSF-HM001-UL)

Product Overview

• Article Name: Human M-CSF/CSF-1 Protein, Ultra Low Endotoxin (CSF-HM001-UL)
• Biozol Catalog Number: KAB-CSF-HM001-UL
• Supplier Catalog Number: CSF-HM001-UL
• Alternative Catalog Number: KAB-CSF-HM001-UL-100UG, KAB-CSF-HM001-UL-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CSF1, CSF-1, MCSF, M-CSF, MGC31930, Lanimostim

Product Description

Recombinant Human M-CSF/CSF-1 Protein is expressed from HEK293 without tag.It contains Glu33-Asn190.

Product Properties

• Molecular Weight: The protein has a predicted MW of 18.40 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result.
• Tag: No Tag
• UniProt: P09603
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Glu33-Asn190
• Target: M-CSF/CSF-1

Images

Human M-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.

Human M-CSF, No Tag immobilized on CM5 Chip can bind Human M-CSF R, hFc Tag with an affinity constant of 2.06 nM as determined in SPR assay (Biacore T200).

Immobilized Human M-CSF, No Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 13.5ng/ml determined by ELISA.

Measured in a cell proliferation assay using RAW264.7 cells.The ED50-for this effect is 1-10 ng/mL.