Human CTLA-4/CD152 Protein (CTL-HM241)

Product Overview

• Article Name: Human CTLA-4/CD152 Protein (CTL-HM241)
• Biozol Catalog Number: KAB-CTL-HM241
• Supplier Catalog Number: CTL-HM241
• Alternative Catalog Number: KAB-CTL-HM241-100UG, KAB-CTL-HM241-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CTLA4, CD152, ICOS, CELIAC3, GRD4, GSE, ALPS5, IDDM12

Product Description

Recombinant Human CTLA-4/CD152 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Lys36-Asp161.

Product Properties

• Molecular Weight: The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: P16410
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Lys36-Asp161
• Target: CTLA-4/CD152

Images

Human CTLA-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CTLA-4 is greater than 95% as determined by SEC-HPLC.

Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA-4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).

Immobilized Human B7-2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.

Immobilized Human B7-1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).