Human DLL4 Protein, Ultra Low Endotoxin (DLL-HM204-UL)

Product Overview

• Article Name: Human DLL4 Protein, Ultra Low Endotoxin (DLL-HM204-UL)
• Biozol Catalog Number: KAB-DLL-HM204-UL
• Supplier Catalog Number: DLL-HM204-UL
• Alternative Catalog Number: KAB-DLL-HM204-UL-100UG, KAB-DLL-HM204-UL-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: DLL-4, MGC126344, notch ligand DLL4, Delta4, Delta-like 4

Product Description

Recombinant Human DLL4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ser27-Pro524.

Product Properties

• Molecular Weight: The protein has a predicted MW of 81.1 kDa. Due to glycosylation, the protein migrates to 82-90 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)
• UniProt: Q9NR61
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ser27-Pro524
• Target: DLL4

Images

Human DLL4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of- Human DLL4 is greater than 95% as determined by SEC-HPLC.

Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 uM as determined in SPR assay (Biacore T200).

Immobilized Human Notch 1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human DLL4, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test).

Immobilized Human DLL4, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 23.5ng/ml determined by ELISA.