Mouse DR3/TNFRSF25 Protein (DR3-MM101)

Product Overview

• Article Name: Mouse DR3/TNFRSF25 Protein (DR3-MM101)
• Biozol Catalog Number: KAB-DR3-MM101
• Supplier Catalog Number: DR3-MM101
• Alternative Catalog Number: KAB-DR3-MM101-100UG, KAB-DR3-MM101-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Mouse
• Alternative Names: APO3, DDR3, DR3, TNFRSF12, WSL, WSL1, TNFRSF25, LARD, TR3, TRAMP, WSL-LR

Product Description

Recombinant Mouse DR3/TNFRSF25 Protein is expressed from HEK293 with His tag at the C-Terminus., It contains Gln31-Met196.

Product Properties

• Molecular Weight: The protein has a predicted MW of 18.79 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: B1AWN9
• Buffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Gln31-Met196
• Target: DR3/TNFRSF25

Images

The purity of Mouse DR3 is greater than 95% as determined by SEC-HPLC.

Mouse DR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Mouse TNFSF15, hFc Tag captured on CM5 Chip via Protein A can bind Mouse DR3, His Tag with an affinity constant of 4.56 nM as determined in SPR assay (Biacore T200).

Immobilized Mouse DR3, His Tag at 1ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).

Loaded Mouse TNFSF15, monomeric Fc Tag on ProA-Biosensor, can bind Mouse DR3, His Tag with an affinity constant of 22.80 nM as determined in BLI assay (Gator).