Human EGF Protein, Ultra Low Endotoxin (EGF-HE001-UL)

Product Overview

• Article Name: Human EGF Protein, Ultra Low Endotoxin (EGF-HE001-UL)
• Biozol Catalog Number: KAB-EGF-HE001-UL
• Supplier Catalog Number: EGF-HE001-UL
• Alternative Catalog Number: KAB-EGF-HE001-UL-100UG, KAB-EGF-HE001-UL-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: HOMG4, EGF, URG

Product Description

Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023.

Product Properties

• Molecular Weight: The protein has a predicted MW of 5.6 kDa same as Bis-Tris PAGE result.
• Tag: No Tag
• UniProt: P01133
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: E.coli
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Asn971-Arg1023
• Target: EGF

Images

The purity of Human EGF is greater than 95% as determined by SEC-HPLC.

Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Human EGF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.56ug/ml determined by ELISA (QC Test).

Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50-for this effect is typically 0.1 - 0.2 ng/mL (QC Test).

Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, No Tag with an affinity constant of 1.33 nM as determined in SPR assay (Biacore T200).