Human EPHB2 Protein (EPH-HM101)

Product Overview

• Article Name: Human EPHB2 Protein (EPH-HM101)
• Biozol Catalog Number: KAB-EPH-HM101
• Supplier Catalog Number: EPH-HM101
• Alternative Catalog Number: KAB-EPH-HM101-100UG, KAB-EPH-HM101-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: DRT, ERK, HEK5, TYRO5, CAPB, EK5, EPHT3, PCBC, Cek5, EphB2, EPHT3MGC87492, Nuk, Qek2, Sek3

Product Description

Recombinant Human EPHB2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val19-Leu543.

Product Properties

• Molecular Weight: The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: P29323
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Val19-Leu543
• Target: EPHB2

Images

Human EPHB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Human EPHB2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.

The purity of Human EPHB2 is greater than 95% as determined by SEC-HPLC.

Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).

Immobilized Human EPHB2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA (QC Test).