Mouse FSTL3 Protein (FTS-MM1L3)

Product Overview

• Article Name: Mouse FSTL3 Protein (FTS-MM1L3)
• Biozol Catalog Number: KAB-FTS-MM1L3
• Supplier Catalog Number: FTS-MM1L3
• Alternative Catalog Number: KAB-FTS-MM1L3-100UG, KAB-FTS-MM1L3-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Mouse
• Alternative Names: FLRG, FLRGFSRP, Follistatin-like 3, FSTL3

Product Description

Recombinant Mouse FSTL3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Val256.

Product Properties

• Molecular Weight: The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: Q9EQC7
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Val24-Val256
• Target: FSTL3

Images

Mouse FSTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Mouse FSTL3 is greater than 95% as determined by SEC-HPLC.

Mouse FSTL3, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A, No tag-with an affinity constant of 53.09 pM as determined in SPR assay (Biacore T200).

Immobilized Human Activin A, No Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).

Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.